Part:BBa_K1810001:Design
Repeating QSP Chain with HlyA secretion Tag
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Hly secretion system has the added requirement that is can only transport unfolded peptide sequence through the membrane; because of this, the string of tripeptides had to be limited in size in order to ensure that folding would not occur and prevent secretion.
Source
The sequence for QSP was designed de novo, as codon optimization for E. coli was taken into account during its construction. The sequence for the OmpC promoter was obtained from the 2011 Missouri iGEM team (BBa_R0082), while the HlyA secretion tag sequence was obtained from the 2011 Brazil-France iGEM team (BBa_K554002). Both of these sequences are naturally found in E. coli, although the HlyA secretion system naturally exists only in pathogenic strains.